NGRL Manchester has updated its popular primer checking tool to incorporate build 132 of dbSNP, released November 2010.

SNPCheck is a unique online tool that enables scientists to easily check their primer sequences for common single nucleotide polymorphisms (SNPs) that may affect the hybridisation of the primer to the target DNA. A sequence divergence of only one nucleotide between primer and target DNA can produce a detectable effect on hybridisation, leading to allelic dropout and potential genotyping failure. SNPCheck is used by scientists around the world to check primers and array probes for SNPs during the design process.
 

The tool aligns submitted primer pair sequences to the human genome reference sequence and then identifies reported SNPs in that region. This latest release of SNPCheck references dbSNP build 132, the most up-to-date database of SNPs world-wide. SNPCheck can be accessed at https://ngrl.manchester.ac.uk/SNPCheckV2/snpcheck.htm