|
MSH2
Mutant CONTROL PLASMID MIX
The MSH2 mut
plasmid mix contains 16 plasmids which contain mutated coding regions
of exons 1-16 as shown below:
Exon
|
Cloned
fragment*
|
Nucleotide
Change * |
Amino
Acid Change* |
1
|
-126 to
IVS1 +256 |
139 C>G |
G47R |
2
|
IVS1 –183
to IVS2 +167 |
283 G>T |
V95F |
3
|
IVS2 –171
to IVS3 +224 |
542 A>T |
N181I |
4
|
IVS3 –325
to IVS4 +213 |
714 T>G |
Y238X |
5
|
IVS4 –114
to IVS5 +177 |
940 C>T |
Q314X |
6
|
IVS5 –187
to IVS6 +234 |
997 T>C |
C333R |
7
|
IVS6 –217
to IVS7 +179 |
1165 C>T |
R389X |
8
|
IVS7 –222
to IVS8 +232 |
1373 T>G |
L458X |
9
|
IVS8 –255
to IVS9 +143 |
1501 A>T |
R501X |
10
|
IVS9 –218
to IVS10 +130 |
1558 G>T |
G520X |
11
|
IVS10 –212
to IVS11 +266 |
1720 C>T |
Q574X |
12
|
IVS11 –180
to IVS12 +254 |
1870 A>T |
I624F |
13
|
IVS12 –183
to IVS13 +187 |
2131 C>T |
R711X |
14
|
IVS13–243
to IVS14 +157 |
2251 G>A |
G751R |
15
|
IVS14 –203
to IVS15 +202 |
2634+1 g>a |
Splice |
16
|
IVS15 –187
to 3020 |
2714 C>G |
T905R |
|
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