NGRL- Wessex

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Projects

Array CGH

Pyrosequencer

Example of a Pyrosequencing assay used for the Analysis of methylation for diagnosis of Prader Willi and Angelmans Syndrome

PCR Amplicon for the analysis of	CpG	sites G-J of the SNRPN gene as quoted in Zeschnigk et al.
Human Molecular Genetics Vol6 387-395

Forward PCR primer:	5´-AGG GAG TTG GGA TTT	TTG TAT T
Reverse PCR primer:	Biotin-5´-CCC CAA ACT ATC TCT TAA AAA AAA C
Sequencing primer:	5´-GTA GAG GTA GGT TGG	(CpG sites G-J)
T is control for bisulfite treatment

Example pyrograms: * PCR amplification bias for unmethylated allele

Last Updated: 7 August, 2008 by G. Watkins