hMLH1 wild type contol plasmid mix

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Non invasive prenatal diagnosis

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Mutation scanning for BRCA1, BRCA2, hMLH1 and MSH2

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RQ-PCR analysis of the BCR-ABL fusion transcript

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hMLH1 wild type CONTROL PLASMID MIX

Reference reagents

hMLH1 wild type CONTROL PLASMID MIX

The hMLH1 wt plasmid mix contains 17 plasmids which contain the wild type coding regions for exons 1-19 flanked by intronic sequence as indicated in the table below:

Exon	Cloned fragment*
1	1 -20 to IVS1+330
2	IVS1-200 to IVS2+183
3	IVS2-235 to IVS3+138
4	IVS3-235 to IVS4+92
5	IVS4-220 to IVS5+140
6	IVS5-141 to IVS6+154
7	IVS6-191 to IVS8+230
8	IVS6-191 to IVS8+230
9	IVS8-187 to IVS9+131
10	IVS9-178 to IVS10+173
11	IVS10-107 to IVS11+179
12	IVS11-166 to IVS12+130
13	IVS12-132 to IVS13+110
14	IVS13-147 to IVS14+158
15	IVS14-207 to IVS15+143
16	IVS15-177 to IVS16+58
17	IVS16-293 to IVS18+79
18	IVS16-293 to IVS18+79
19	IVS18-74 to 2434

Last Updated: July 24, 2008 by G. Watkins.