|
This is not intended to be a comprehensive list of extraction protocols.
In view of the current requirements the evaluation list has been focused
at high yield (large volume) extractions.
| Company |
Chemistry |
Automation
options* |
Basis |
Max
blood
volume
(ml)
|
Max
yield
(ug/ml)
blood
|
Evaluation** |
| Systems |
| ABI |
|
6100Nucleic
Acid PrepStation |
Solid
phase extraction |
0.15 |
40 |
NGRL (Wessex) |
| AutoGen |
Automated
nucleic acid isolation system |
NA-3000EU |
Salt
precipitation |
7.00 |
30 |
NGRL
(Wessex) |
| Chemagen |
Chemagic |
Chemagen
Seperation module 1 |
paramagnetic
beads |
20.00 |
40 |
NGRL
(Wessex) |
Gentra
|
Puregene® |
AUTOPURE
LS® automated
DNA extractor |
Salt
precipitation |
20.00 |
50 |
NGRL
(Manchester) |
| Qiagen |
|
EZ1 |
paramagnetic
beads |
0.35 |
30 |
NGRL
(Wessex) |
| Roche |
|
MagNA Pure Compact |
paramagnetic
beads |
1.00 |
40 |
NGRL
(Wessex) |
Chemistries
suitable for automation |
Chemagen
|
Chemagic1K |
Chemagen
Seperation module 1, TECAN, PSS Magtration |
paramagnetic
beads |
1.00 |
30 |
NGRL
(Wessex) |
Chemagen
|
Chemagic5K
|
Chemagen
Seperation module 1,TECAN,
PSS Magtration |
paramagnetic
beads |
5.00 |
30 |
NGRL
(Wessex) |
| Chemagen |
Chemagic10K |
Chemagen
Seperation module 1,TECAN,
PSS Magtration |
paramagnetic
beads |
10.00 |
30 |
NGRL
(Wessex) |
| DRI |
CST |
TECAN |
paramagnetic
beads |
|
40 |
NGRL
(Wessex) |
Macherey
Nagel
|
NucleoBond
CB |
TECAN |
anion
exchange |
20.00 |
20 |
NGRL
(Wessex) |
Macherey
Nagel
|
NucleoSpin Blood
L |
TECAN |
silica
membrane |
2.00 |
30 |
NGRL (Wessex)
|
Macherey
Nagel
|
NucleoSpin
Blood XL |
TECAN |
silica
membrane |
10.00 |
30 |
NGRL
(Wessex) |
| Promega |
Wizard® Genomic
DNA Kit |
Biomek
FX, TECAN |
precipitation |
10.00 |
50 |
NGRL
(Wessex) |
*Automation options given are links between companies
as stated by them. Many of the above chemistries could be automated
on
other platforms.
**where more than one chemistry from one supplier is listed
only a representative sample of kits available will be evaluated.
Evaluation
report – “Automated DNA Extraction Methodologies” (download)
Useful links :
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